Description
Description: This assay employs a two-site sandwich ELISA to quantitate XYLB in samples. An antibody specific for XYLB has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any XYLB present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for XYLB is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of XYLB bound in the initial step. The color development is stopped and the intensity of the color is measured.
Overview: XYLB shares 22% sequence identity with Hemophilus influenzae xylulokinase, and even higher identity to other gene products in C.elegans (45%) and yeast (31-35%), which are thought to belong to a family of enzymes that include fucokinase, gluconokinase, glycerokinase and xylulokinase. These proteins play important roles in energy metabolism.
The 3p22-p21.3 chromosomal region is one of 3 regions of 3p that is commonly deleted in various carcinomas. The XYLB gene contains 18 exons and spans approximately 28 kb. Northern blot analysis revealed that XYLB was expressed as a 2.3-kb major transcript in all tissues tested. A less abundant 1.8-kb mRNA was detected in heart and skeletal muscle.XYLB gene is located between the OCTL2 and ActRIIB genes on 3p22-p21.3